Disposing of biohazards in the right way

Question 1 

1. Most scientists who use DNA      microarrays obtain them

 

from companies that mass produce them

 

from the government

 

by making them in their labs

 

by exploiting ambitious graduate   students

4 points

Question 2 

1. “In this study, cancerous and      normal skin tissue samples were excised with a scalpel. Each skin sample      was then placed into a sample tube along with solvent, shaken on a vortex      and centrifuged. When the sample tubes came out of the centrifuge, there      was a top layer and a bottom layer. For our purposes, we went on to use      ______________ for further processing.”

 

only the top layer

 

only the bottom layer

 

a mixture of top and bottom layers

 

none of the options listed

4 points

Question 3 

1. The purpose of the vortex      instrument is to

 

vigorously shake the tissue sample in   the solvent so that it dissolves.

 

spin the tissue/solvent mixture   around to separate the solids

 

cause the mRNA to bind to the pellets

 

dispose of biohazardous waste safely

3 points

Question 4 

1. “Once the RNA is isolated      from the DNA, proteins and other materials, the solvent/RNA mixture is run      through a column of all beads. Each bead has several short strands of      polyT RNA sticking out of it. In this step:”

 

“mRNA sticks to the beads, while   rRNA and tRNA run right through the column”

 

“tRNA sticks to the beads, while   mRNA and rRNA run right through the column”

 

“rRNA and tRNA stick to the   beads, while mRNA runs right through the column”

 

none of the options listed

4 points

Question 5 

1. “Once the mRNA is isolated,      you make a DNA copy of it using by adding polyT primers, labeled DNA      nucleotides, and an enzyme known as:”

 

reverse transcriptase

 

tyrosine hydroxylase

 

alcohol dehydrogenase

 

ATP kinase

4 points

Question 6 

1. A DNA copy of a mRNA transcript is      known as

 

complementary DNA (abbreviated cDNA)

 

copied DNA (abbreviated cDNA)

 

mitochondrial DNA (abbreviated mtDNA)

 

recombinant DNA (abbreviated rDNA)

3 points